A detection method for the capture of genomic signatures: From disease diagnosis to genome editing

Methods Enzymol. 2021:661:251-282. doi: 10.1016/bs.mie.2021.08.012. Epub 2021 Sep 28.


Variations in the genetic information originate from errors during DNA replication, error-prone repair of DNA damages, or genome editing. The most common approach to detect changes in DNA sequences employs sequencing technologies. However, they remain expensive and time-consuming, limiting their utility for routine laboratory experiments. We recently developed DinucleoTidE Signature CapTure (DTECT). DTECT is a marker-free and versatile detection method that captures targeted dinucleotide signatures resulting from the digestion of genomic amplicons by the type IIS restriction enzyme AcuI. Here, we describe the DTECT protocol to identify mutations introduced by CRISPR-based precision genome editing technologies or resulting from genetic variation. DTECT enables accurate detection of mutations using basic laboratory equipment and off-the-shelf reagents with qualitative or quantitative capture of signatures.

Keywords: Base editing prime editing; CRISPR; DTECT; Detection method; Genetic variation; Pathogenic variants; Precision genome editing; Signature capture.

MeSH terms

  • CRISPR-Cas Systems* / genetics
  • Gene Editing* / methods
  • Genome
  • Genomics
  • Mutation