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. 2021 Nov 3:2021:8311588.
doi: 10.1155/2021/8311588. eCollection 2021.

Systematic Study on a Quantitative Analysis of Multicomponents by Single Marker (QAMS) Method for Simultaneous Determination of Eight Constituents in Pneumonia Mixture by UPLC-MS/MS

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Systematic Study on a Quantitative Analysis of Multicomponents by Single Marker (QAMS) Method for Simultaneous Determination of Eight Constituents in Pneumonia Mixture by UPLC-MS/MS

Haibo Zhang et al. J Anal Methods Chem. .

Abstract

Pneumonia mixture was formulated and is available to treat children acute pneumonia and acute bronchitis in our hospital for nearly forty years, but there are few studies of its quality evaluation or control. In this paper, a new strategy for quality evaluation of pneumonia mixture was explored and verified through qualitative and quantitative analyses of multicomponents by single marker (QAMS) by UPLC-MS/MS. Baicalein was selected as an internal reference, and the relative correction factors (RCFs) and the relative retention time (RRT) of (R, S)-goitrin, amygdalin, chlorogenic acid, pseudoephedrine hydrochloride, ephedrine hydrochloride, ammonium glycyrrhizinate, and baicalin were established. The robustness and durability of the QAMS method were investigated. RCF values calculated by the average (AVG) method and linear regression (LRG) method had good repeatability and were acceptable for quantitative analysis, and the RTT combined with the exact masses of precursor and fragment ions and their abundance could be adopted for accurately positioning the chromatographic peak of the eight constituents. The consistency and feasibility of the QAMS method were verified by comparing the contents of the seven components calculated by a classic and validated external standard method (ESM) with those of the QAMS method, which reduces analytical cost and time of detection and avoids the problem of the diversity and large quantity of reference standards. The results demonstrated that the QAMS method developed in this paper could provide a new, alternative, and promising method to comprehensively and effectively determine multicomponents and control the quality of pneumonia mixture or even a group of similar medicines.

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Conflict of interest statement

All authors have confirmed that there are no conflicts of interest in the publication of this study.

Figures

Figure 1
Figure 1
The chemical structures of the investigated compounds: (a) (R, S)-goitrin, (b) ephedrine hydrochloride, (c) pseudoephedrine hydrochloride, (d) chlorogenic acid, (e) amygdalin, (f) baicalein, (g) ammonium glycyrrhizinate, and (h) baicalin.
Figure 2
Figure 2
Typical UPLC-MS/MS analysis MRM chromatograms of the mixed standard solution: A (R, S)-goitrin, B amygdalin, C chlorogenic acid, D pseudoephedrine hydrochloride, E ephedrine hydrochloride, F ammonium glycyrrhizinate, G baicalein, and H baicalin.
Figure 3
Figure 3
Typical UPLC-MS/MS analysis MRM chromatograms of the sample solution: A (R, S)-goitrin, B amygdalin, C chlorogenic acid, D pseudoephedrine hydrochloride, E ephedrine hydrochloride, F ammonium glycyrrhizinate, G baicalein, and H baicalin.
Figure 4
Figure 4
Bar charts of the SMDs. The RCFs were calculated by AVG (a) and LRG (b) methods.

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