SARS-CoV-2 deregulates the vascular and immune functions of brain pericytes via Spike protein

Neurobiol Dis. 2021 Dec;161:105561. doi: 10.1016/j.nbd.2021.105561. Epub 2021 Nov 13.

Abstract

Coronavirus disease 19 (COVID-19) is a respiratory illness caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). COVID-19 pathogenesis causes vascular-mediated neurological disorders via elusive mechanisms. SARS-CoV-2 infects host cells via the binding of viral Spike (S) protein to transmembrane receptor, angiotensin-converting enzyme 2 (ACE2). Although brain pericytes were recently shown to abundantly express ACE2 at the neurovascular interface, their response to SARS-CoV-2 S protein is still to be elucidated. Using cell-based assays, we found that ACE2 expression in human brain vascular pericytes was increased upon S protein exposure. Pericytes exposed to S protein underwent profound phenotypic changes associated with an elongated and contracted morphology accompanied with an enhanced expression of contractile and myofibrogenic proteins, such as α-smooth muscle actin (α-SMA), fibronectin, collagen I, and neurogenic locus notch homolog protein-3 (NOTCH3). On the functional level, S protein exposure promoted the acquisition of calcium (Ca2+) signature of contractile ensheathing pericytes characterized by highly regular oscillatory Ca2+ fluctuations. Furthermore, S protein induced lipid peroxidation, oxidative and nitrosative stress in pericytes as well as triggered an immune reaction translated by activation of nuclear factor-kappa-B (NF-κB) signaling pathway, which was potentiated by hypoxia, a condition associated with vascular comorbidities that exacerbate COVID-19 pathogenesis. S protein exposure combined to hypoxia enhanced the production of pro-inflammatory cytokines involved in immune cell activation and trafficking, namely macrophage migration inhibitory factor (MIF). Using transgenic mice expressing the human ACE2 that recognizes S protein, we observed that the intranasal infection with SARS-CoV-2 rapidly induced hypoxic/ischemic-like pericyte reactivity in the brain of transgenic mice, accompanied with an increased vascular expression of ACE2. Moreover, we found that SARS-CoV-2 S protein accumulated in the intranasal cavity reached the brain of mice in which the nasal mucosa is deregulated. Collectively, these findings suggest that SARS-CoV-2 S protein impairs the vascular and immune regulatory functions of brain pericytes, which may account for vascular-mediated brain damage. Our study provides a better understanding for the mechanisms underlying cerebrovascular disorders in COVID-19, paving the way to develop new therapeutic interventions.

Keywords: COVID-19; Cerebrovascular disorders; Inflammation; Myofibrogenic transition; Neurovascular interface; Pericytes; SARS-CoV-2 S protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / metabolism
  • Angiotensin-Converting Enzyme 2 / drug effects
  • Angiotensin-Converting Enzyme 2 / genetics
  • Angiotensin-Converting Enzyme 2 / metabolism*
  • Animals
  • Brain / blood supply
  • Brain / metabolism*
  • COVID-19 / metabolism*
  • COVID-19 / physiopathology
  • Calcium Signaling
  • Collagen Type I / metabolism
  • Fibronectins / metabolism
  • Humans
  • Hypoxia / metabolism*
  • Hypoxia-Ischemia, Brain / metabolism*
  • Hypoxia-Ischemia, Brain / physiopathology
  • Inflammation / metabolism*
  • Lipid Peroxidation / drug effects
  • Lipid Peroxidation / genetics
  • Macrophage Migration-Inhibitory Factors / drug effects
  • Macrophage Migration-Inhibitory Factors / metabolism
  • Mice
  • Mice, Transgenic
  • Muscle, Smooth, Vascular / cytology
  • Muscle, Smooth, Vascular / metabolism
  • Myocytes, Smooth Muscle / cytology
  • Myocytes, Smooth Muscle / metabolism
  • Myofibroblasts
  • NF-kappa B / drug effects
  • NF-kappa B / metabolism
  • Nasal Mucosa
  • Nitrosative Stress
  • Oxidative Stress
  • Pericytes / cytology
  • Pericytes / drug effects
  • Pericytes / metabolism*
  • Phenotype
  • Receptor, Notch3 / metabolism
  • Receptors, Coronavirus / drug effects
  • Receptors, Coronavirus / genetics
  • Receptors, Coronavirus / metabolism
  • SARS-CoV-2 / metabolism*
  • Spike Glycoprotein, Coronavirus / metabolism*
  • Spike Glycoprotein, Coronavirus / pharmacology

Substances

  • ACTA2 protein, human
  • Actins
  • Collagen Type I
  • Fibronectins
  • Macrophage Migration-Inhibitory Factors
  • NF-kappa B
  • NOTCH3 protein, human
  • Receptor, Notch3
  • Receptors, Coronavirus
  • Spike Glycoprotein, Coronavirus
  • spike protein, SARS-CoV-2
  • ACE2 protein, human
  • Angiotensin-Converting Enzyme 2