Sensitive identification of neoantigens and cognate TCRs in human solid tumors

Nat Biotechnol. 2022 May;40(5):656-660. doi: 10.1038/s41587-021-01072-6. Epub 2021 Nov 15.


The identification of patient-specific tumor antigens is complicated by the low frequency of T cells specific for each tumor antigen. Here we describe NeoScreen, a method that enables the sensitive identification of rare tumor (neo)antigens and of cognate T cell receptors (TCRs) expressed by tumor-infiltrating lymphocytes. T cells transduced with tumor antigen-specific TCRs identified by NeoScreen mediate regression of established tumors in patient-derived xenograft mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Neoplasm / genetics
  • CD8-Positive T-Lymphocytes
  • Humans
  • Lymphocytes, Tumor-Infiltrating
  • Mice
  • Neoplasms* / genetics
  • Neoplasms* / therapy
  • Receptors, Antigen, T-Cell* / genetics
  • T-Lymphocytes


  • Antigens, Neoplasm
  • Receptors, Antigen, T-Cell