Extracellular Vesicles from Human Urine-Derived Stem Cells Ameliorate Particulate Polyethylene-Induced Osteolysis

Hui Li; Xiao-Lei Fan; Yi-Nan Wang; Wei Lu; Haoyi Wang; Runzhi Liao; Min Zeng; Jun-Xiao Yang; Yihe Hu; Jie Xie

doi:10.2147/IJN.S325646

Extracellular Vesicles from Human Urine-Derived Stem Cells Ameliorate Particulate Polyethylene-Induced Osteolysis

Int J Nanomedicine. 2021 Nov 6:16:7479-7494. doi: 10.2147/IJN.S325646. eCollection 2021.

Authors

Hui Li^#^{1

2}, Xiao-Lei Fan^#^{1

2}, Yi-Nan Wang^{1

2}, Wei Lu^{1

2}, Haoyi Wang^{1

2}, Runzhi Liao^{1

2}, Min Zeng^{1

2}, Jun-Xiao Yang^{1

2}, Yihe Hu^{1

2}, Jie Xie^{1

2}

Affiliations

¹ Department of Orthopedics, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.
² Hunan Engineering Research Center of Biomedical Metal and Ceramic Implants, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.

^# Contributed equally.

Abstract

Purpose: Wear debris particle-induced periprosthetic osteolysis is a severe complication of total joint replacement that results in aseptic loosening and subsequent arthroplasty failure. No effective therapeutic agents or drugs have been approved to prevent or treat osteolysis; thus, revision surgery is often needed. Extracellular vesicles (EVs) are vital nanosized regulators of intercellular communication that can be directly applied to promote tissue repair and regeneration. In this study, we assessed the therapeutic potential of EVs from human urine-derived stem cells (USCs) (USC-EVs) in preventing ultrahigh-molecular-weight polyethylene (UHMWPE) particle-induced osteolysis.

Methods: USCs were characterized by measuring induced multipotent differentiation and flow cytometry. USC-EVs were isolated and characterized using transmission electron microscopy (TEM), dynamic light scattering (DLS) and Western blotting. RAW264.7 cells and bone marrow mesenchymal stem cells (BMSCs) were cultured with USC-EVs to verify osteoclast differentiation and osteoblast formation, respectively, in vitro. The effects of USC-EVs were investigated on a UHMWPE particle-induced murine calvarial osteolysis model by assessing bone mass, the inflammatory reaction, and osteoblast and osteoclast formation.

Results: USCs differentiated into osteogenic, adipogenic and chondrogenic cells in vitro and were positive for CD44, CD73, CD29 and CD90 but negative for CD34 and CD45. USC-EVs exhibited a cup-like morphology with a double-layered membrane structure and were positive for CD63 and TSG101 and negative for calnexin. In vitro, USC-EVs promoted the osteogenic differentiation of BMSCs and reduced proinflammatory factor production and osteoclastic activity in RAW264.7 cells. In vivo, local injection of USC-EVs around the central sites of the calvaria decreased inflammatory cytokine generation and osteolysis compared with the control groups and significantly increased bone formation.

Conclusion: Based on our findings, USC-EVs prevent UHMWPE particle-induced osteolysis by decreasing inflammation, suppressing bone resorption and promoting bone formation.

Keywords: UHMWPE; anti-inflammatory; extracellular vesicles; urine-derived stem cells; wear particle-induced osteolysis.

MeSH terms

Animals
Extracellular Vesicles*
Humans
Mice
Osteoclasts
Osteogenesis
Osteolysis* / chemically induced
Polyethylene
Stem Cells

Substances

Polyethylene

Grants and funding

This work was supported by the National Natural Science Foundation of China (Grant No. 81974339) and the Science and Technology Plan Project of Hunan Province (Grant No. 2019JJ40499).