Cloning and expression of T4 DNA polymerase

Proc Natl Acad Sci U S A. 1987 Oct;84(20):7000-4. doi: 10.1073/pnas.84.20.7000.


The structural gene coding for bacteriophage T4 DNA polymerase (gene 43) has been cloned into inducible plasmid vectors, which provide a source for obtaining large amounts of this enzyme after induction. The T4 DNA polymerase produced in this fashion was purified by an innovative three-step procedure and was fully active.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Genes
  • Genes, Viral*
  • Genetic Vectors
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Fusion Proteins / genetics
  • T-Phages / enzymology
  • T-Phages / genetics*
  • Viral Proteins / biosynthesis
  • Viral Proteins / genetics*


  • Recombinant Fusion Proteins
  • Viral Proteins