Identification of proximal SUMO-dependent interactors using SUMO-ID

Nat Commun. 2021 Nov 18;12(1):6671. doi: 10.1038/s41467-021-26807-6.


The fast dynamics and reversibility of posttranslational modifications by the ubiquitin family pose significant challenges for research. Here we present SUMO-ID, a technology that merges proximity biotinylation by TurboID and protein-fragment complementation to find SUMO-dependent interactors of proteins of interest. We develop an optimized split-TurboID version and show SUMO interaction-dependent labelling of proteins proximal to PML and RANGAP1. SUMO-dependent interactors of PML are involved in transcription, DNA damage, stress response and SUMO modification and are highly enriched in SUMO Interacting Motifs, but may only represent a subset of the total PML proximal proteome. Likewise, SUMO-ID also allow us to identify interactors of SUMOylated SALL1, a less characterized SUMO substrate. Furthermore, using TP53 as a substrate, we identify SUMO1, SUMO2 and Ubiquitin preferential interactors. Thus, SUMO-ID is a powerful tool that allows to study the consequences of SUMO-dependent interactions, and may further unravel the complexity of the ubiquitin code.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • GTPase-Activating Proteins / metabolism
  • HEK293 Cells
  • Humans
  • Promyelocytic Leukemia Protein / metabolism
  • Protein Binding
  • Protein Interaction Mapping / methods*
  • Protein Interaction Maps*
  • Protein Processing, Post-Translational*
  • SUMO-1 Protein / metabolism
  • Small Ubiquitin-Related Modifier Proteins / metabolism*
  • Sumoylation*
  • Transcription Factors / metabolism
  • Tumor Suppressor Protein p53 / metabolism
  • Ubiquitin / metabolism


  • GTPase-Activating Proteins
  • Promyelocytic Leukemia Protein
  • RANGAP1 protein, human
  • SALL1 protein, human
  • SUMO-1 Protein
  • SUMO1 protein, human
  • SUMO2 protein, human
  • Small Ubiquitin-Related Modifier Proteins
  • Transcription Factors
  • Tumor Suppressor Protein p53
  • Ubiquitin