In Vivo Imaging of Tfh Cells

Sandra Nakandakari-Higa; Johanne T Jacobsen

doi:10.1007/978-1-0716-1736-6_2

In Vivo Imaging of Tfh Cells

Methods Mol Biol. 2022:2380:15-27. doi: 10.1007/978-1-0716-1736-6_2.

Authors

Sandra Nakandakari-Higa¹, Johanne T Jacobsen²

Affiliations

¹ Laboratory of Lymphocyte Dynamics, The Rockefeller University, New York, NY, USA.
² Laboratory of Lymphocyte Dynamics, The Rockefeller University, New York, NY, USA. jjacobsen@rockefeller.edu.

PMID: 34802118
DOI: 10.1007/978-1-0716-1736-6_2

Abstract

Germinal centers (GCs) are microanatomical structures in secondary lymphoid organs where B cells undergo affinity maturation for antigen during the course of an immune response. This process is driven by a subset of T cells termed T follicular helper cells (Tfh) that through a multistep process gain access to the GC niche within the B cell follicle. This protocol details how to study Tfh behavior in vivo, on a single cell level, using two-photon intravital microscopy of the murine popliteal lymph node.

Keywords: Germinal centers; In vivo imaging; Intravital microscopy; T follicular helper cells; T-B interactions.

MeSH terms

Animals
B-Lymphocytes
Germinal Center
Lymph Nodes
Mice
T Follicular Helper Cells*
T-Lymphocytes, Helper-Inducer