Optimized workflow for human PBMC multiomic immunosurveillance studies

STAR Protoc. 2021 Nov 5;2(4):100900. doi: 10.1016/j.xpro.2021.100900. eCollection 2021 Dec 17.


Deep immune profiling is essential for understanding the human immune system in health and disease. Successful biological interpretation of this data requires consistent laboratory processing with minimal batch-to-batch variation. Here, we detail a robust pipeline for the profiling of human peripheral blood mononuclear cells by both high-dimensional flow cytometry and single-cell RNA-seq. These protocols reduce batch effects, generate reproducible data, and increase throughput. For complete details on the use and execution of this protocol, please refer to Savage et al. (2021).

Keywords: Cell Biology; Flow Cytometry/Mass Cytometry; Immunology; Molecular Biology; RNAseq; Sequencing; Single Cell.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Computational Biology
  • Flow Cytometry / methods*
  • Humans
  • Leukocytes, Mononuclear* / chemistry
  • Leukocytes, Mononuclear* / classification
  • Leukocytes, Mononuclear* / cytology
  • Leukocytes, Mononuclear* / immunology
  • Monitoring, Immunologic / methods*
  • Sequence Analysis, RNA
  • Single-Cell Analysis / methods*