Expression of Cyclin D1 gene in ovarian cancer and effect of silencing its expression on ovarian cancer cells based on the Oncomine database

Li-Li Quan; Jin-Yang Liu; Li-Xia Qu; Hui La; Hai-Li Wang; Xiao-Xia Chen; Na Wang; Zhen-Zhi Wei

doi:10.1080/21655979.2021.2000225

Expression of Cyclin D1 gene in ovarian cancer and effect of silencing its expression on ovarian cancer cells based on the Oncomine database

Bioengineered. 2021 Dec;12(2):9290-9300. doi: 10.1080/21655979.2021.2000225.

Authors

Li-Li Quan¹, Jin-Yang Liu¹, Li-Xia Qu¹, Hui La¹, Hai-Li Wang², Xiao-Xia Chen¹, Na Wang¹, Zhen-Zhi Wei¹

Affiliations

¹ Department of Gynecology, Sanmenxia Central Hospital of Henan University of Science and Technology, Sanmenxia, Henan Province, China.
² Department of Anesthesiology, Sanmenxia Central Hospital of Henan University of Science and Technology, Sanmenxia, Henan Province, China.

Abstract

We aimed to analyze the expression of Cyclin D1 (CCND1) gene in ovarian cancer and the influence of silencing its expression on ovarian cancer cells based on the Oncomine database. The expression of CCND1 gene in ovarian cancer was analyzed by utilizing the relevant information in different tumors and Oncomine database. The correlation between CCDN1 expression level and prognosis of ovarian cancer was analyzed by the online database Kaplan-Meier (kmplot.com). The expression of CCND1 gene in ovarian cancer and the effect of silencing its expression on cancer cells were analyzed by cell experiments. After mining and comprehensively analyzing 7 studies on the differential expression of CCND1 gene in ovarian cancer tissue and normal ovarian tissue included in the Oncomine database, it was found that the median value of CCND1 gene ranked 218.0 (P = 8.03 × 10^-6) among all differentially expressed genes, suggesting that CCND1 gene expression in ovarian cancer tissue was higher than that in normal ovarian tissue. Adib Ovarian, Bonome Ovarian and Hendrix Ovarian microarrays revealed that the expression of CCND1 gene in ovarian cancer tissue was significantly higher than that in normal ovarian tissue (P < 0.05). Kaplan-Meier Plotter database showed that the overall survival and progression-free survival of ovarian cancer patients with high CCND1 expression were significantly shorter than those of patients with low CCND1 expression (P < 0.05). The expression levels of CCND1 gene in normal ovarian epithelial cells and SKOV3 ovarian cancer cells were detected by RT-PCR. The expression of CCND1 gene was significantly higher in SKOV3 group than that in control group (P < 0.01). Flow cytometry revealed that the percentage of cells in G₀/G₁ phase was significantly higher, while that in S phase was lower in SKOV3 + siCCND1 group than the values of SKOV3 and SKOV3 + siNC groups (P < 0.05). The apoptosis rate of ovarian cancer cells was significantly higher in SKOV3 + siCCND1 group than those of SKOV3 and SKOV3 + siNC groups (P < 0.01). CCND1 gene is highly expressed in ovarian cancer tissue and related to prognosis. Preoperative evaluation of CCND1 gene expression in ovarian cancer patients may benefit the assessment of risk and prognosis.

Keywords: Cyclin D1; Oncomine; ovarian cancer; silencing.

MeSH terms

Apoptosis / genetics
Cell Cycle / genetics
Cell Line, Tumor
Cyclin D1 / genetics*
Cyclin D1 / metabolism
Databases, Genetic*
Epithelial Cells / metabolism
Female
Gene Expression Regulation, Neoplastic*
Gene Regulatory Networks
Gene Silencing*
Humans
Ovarian Neoplasms / genetics*
Ovarian Neoplasms / mortality
Ovarian Neoplasms / pathology
Survival Rate

Substances

CCND1 protein, human
Cyclin D1

Grants and funding

The author(s) reported there is no funding associated with the work featured in this article.