Performance of the eazyplex® BloodScreen GN as a simple and rapid molecular test for identification of Gram-negative bacteria from positive blood cultures

Eur J Clin Microbiol Infect Dis. 2022 Mar;41(3):489-494. doi: 10.1007/s10096-021-04383-3. Epub 2021 Nov 22.


The LAMP-based eazyplex® BloodScreen GN was evaluated for the detection of frequent Gram-negatives directly from positive blood culture (BC) bottles. A total of 449 BCs were analyzed. Sensitivities and specificities were 100% and 100% for Escherichia coli, 95.7% and 100% for Klebsiella pneumoniae, 100% and 100% for blaCTX-M, 100% and 100% for Klebsiella oxytoca, 100% and 99% for Proteus mirabilis, and 100% and 99.8% for Pseudomonas aeruginosa, respectively. The time to result ranged from 8 to 16 min, plus about 6 min for sample preparation. The eazyplex® BloodScreen GN is a reliable molecular assay for rapid BC testing.

Keywords: Blood culture; Gram-negative bacilli; LAMP; Rapid diagnostics; Sepsis.

MeSH terms

  • Bacteremia* / diagnosis
  • Blood Culture
  • Escherichia coli Infections*
  • Gram-Negative Bacteria / genetics
  • Humans