The identity of Neoperla sinensis Chu, 1928 is confirmed in this paper. Two new species of Neoperla, N. huangshana Huo, Du Zhu, sp. nov. and N. pangi Huo, Du Zhu, sp. nov. are proposed from Anhui Province, southeast China, closely related to N. sinensis. Neoperla anjiensis Yang Yang, 1998 is newly recorded in Anhui Province.