Comparative proteomics suggests the mode of action of a novel molluscicide against the invasive apple snail Pomacea canaliculata, intermediate host of Angiostrongylus cantonensis

Yingxiang Xu; Weisi Wang; Junmin Yao; Minli Yang; Yunhai Guo; Zhuohui Deng; Qiang Mao; Shizhu Li; Liping Duan

doi:10.1016/j.molbiopara.2021.111431

Comparative proteomics suggests the mode of action of a novel molluscicide against the invasive apple snail Pomacea canaliculata, intermediate host of Angiostrongylus cantonensis

Mol Biochem Parasitol. 2022 Jan:247:111431. doi: 10.1016/j.molbiopara.2021.111431. Epub 2021 Nov 20.

Authors

Yingxiang Xu¹, Weisi Wang², Junmin Yao², Minli Yang³, Yunhai Guo², Zhuohui Deng⁴, Qiang Mao⁴, Shizhu Li², Liping Duan⁵

Affiliations

¹ College of Chemistry and Materials Science, Shanghai Normal University, Shanghai, 200234, China; NHC Key Laboratory of Parasite and Vector Biology, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Centre for Tropical Diseases, Shanghai, 200025, China.
² NHC Key Laboratory of Parasite and Vector Biology, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Centre for Tropical Diseases, Shanghai, 200025, China.
³ College of Chemistry and Materials Science, Shanghai Normal University, Shanghai, 200234, China. Electronic address: yml_nx@shnu.edu.cn.
⁴ Guangdong Provincial Center for Disease Control and Prevention, WHO Collaborating Centre for Surveillance, Research and Training of Emerging Infectious Diseases, Guangzhou, 511430, China.
⁵ College of Chemistry and Materials Science, Shanghai Normal University, Shanghai, 200234, China; NHC Key Laboratory of Parasite and Vector Biology, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Centre for Tropical Diseases, Shanghai, 200025, China. Electronic address: duanlp@nipd.chinacdc.cn.

PMID: 34813866
DOI: 10.1016/j.molbiopara.2021.111431

Abstract

Angiostrongylus cantonensis is a zoonotic parasitic nematode that is the most common cause of human eosinophilic meningitis. The invasive apple snail Pomacea canaliculata is an important intermediate host of A. cantonensis and contributes to its spread. P. canaliculata control will help prevent its invasion and transmission of A. cantonensis. The new molluscicide PBQ (1-(4-chlorophenyl)-3-(pyridin-3-yl)urea) exhibits great potency against P. canaliculata and has low toxicity against mammals and non-target aquatic organisms. We studied the mode of action of PBQ using TMT-based comparative quantitative proteomics analysis between PBQ-treated and control P. canaliculata snails. A total of 3151 proteins were identified, and 245 of these proteins were significantly differentially expressed with 135 downregulated and 110 upregulated. GO and KEGG enrichment analyses identified GO terms and KEGG pathways involved in de novo purine biosynthesis, ribosome components and translation process were significantly enriched and downregulated. The results indicated that PBQ treatment had substantial effects on the synthesis of genetic material, translation process, and protein synthesis of P. canaliculata and were likely the main cause of snail mortality.

Keywords: Angiostrongylus cantonensis; Invasive species; Molluscicide; Pomacea canaliculata; Proteomics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Angiostrongylus cantonensis* / genetics
Animals
Gastropoda*
Humans
Mammals
Proteomics
Strongylida Infections*