Enhancing cofactor regeneration of cyanobacteria for the light-powered synthesis of chiral alcohols

Jianhua Fan; Yinghui Zhang; Ping Wu; Xiaoyan Zhang; Yunpeng Bai

doi:10.1016/j.bioorg.2021.105477

Enhancing cofactor regeneration of cyanobacteria for the light-powered synthesis of chiral alcohols

Bioorg Chem. 2022 Jan:118:105477. doi: 10.1016/j.bioorg.2021.105477. Epub 2021 Nov 10.

Authors

Jianhua Fan¹, Yinghui Zhang¹, Ping Wu¹, Xiaoyan Zhang², Yunpeng Bai³

Affiliations

¹ State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China; Department of Applied Biology, East China University of Science and Technology, Shanghai 200237, PR China.
² State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China; Department of Bioengineering, East China University of Science and Technology, Shanghai 200237, PR China.
³ State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China; Department of Bioengineering, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: ybai@ecust.edu.cn.

PMID: 34814084
DOI: 10.1016/j.bioorg.2021.105477

Abstract

Cyanobacteria Synechocystis sp. PCC 6803 was exploited as green cell factory for light-powered asymmetric synthesis of aromatic chiral alcohols. The effect of temperature, light, substrate and cell concentration on substrate conversions were investigated. Under the optimal condition, a series of chiral alcohols were synthesized with conversions up to 95% and enantiomer excess (ee) > 99%. We found that the addition of Na₂S₂O₃ and Angeli's Salt increased the NADPH content by 20% and 25%, respectively. As a result, the time to reach 95% substrate conversion was shortened by 12 h, which demonstrated that the NADPH regeneration and hence the reaction rates can be regulated in cyanobacteria. This blue-green algae based biocatalysis showed its potential for chiral compounds production in future.

Keywords: Asymmetric synthesis; Biocatalysis; Chiral alcohol; Cofactor regeneration; Cyanobacteria.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alcohols / chemistry
Alcohols / metabolism*
Light*
Molecular Structure
NADP / biosynthesis*
NADP / chemistry
Synechocystis / chemistry*
Synechocystis / metabolism

Substances

Alcohols
NADP