Two simple but effective turn-on benzothiazole-based fluorescent probes for detecting hydrogen sulfide in real water samples and HeLa cells

Tian-Zhen Liu; Xiao-Ling Cui; Wen-Long Sun; Jun-Ying Miao; Bao-Xiang Zhao; Zhao-Min Lin

doi:10.1016/j.aca.2021.339225

Two simple but effective turn-on benzothiazole-based fluorescent probes for detecting hydrogen sulfide in real water samples and HeLa cells

Anal Chim Acta. 2022 Jan 2:1189:339225. doi: 10.1016/j.aca.2021.339225. Epub 2021 Oct 30.

Authors

Tian-Zhen Liu¹, Xiao-Ling Cui², Wen-Long Sun¹, Jun-Ying Miao², Bao-Xiang Zhao³, Zhao-Min Lin⁴

Affiliations

¹ Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, PR China.
² Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Science, Shandong University, Qingdao, 266237, PR China.
³ Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, PR China. Electronic address: bxzhao@sdu.edu.cn.
⁴ Institute of Medical Science, The Second Hospital of Shandong University, Jinan, 250033, PR China. Electronic address: linzhaomin@sdu.edu.cn.

PMID: 34815049
DOI: 10.1016/j.aca.2021.339225

Abstract

Two simple turn-on fluorescent probes, containing a benzothiazole and the 2,4-dinitrobenzenesulfonyl group, were designed for detecting H₂S. Two probes exhibited good selectivity and high sensitivity, which were applied to detect the H₂S in real water samples. Probe P2 with a positive charge had better solubility than probe P1 in water; therefore, probe P2 was successfully applied to detect both the endogenous and exogenous H₂S in lysosomes of living HeLa cells.

Keywords: Cell image; H(2)S; Lysosome-targeted; PET; Turn-on fluorescent probes; Water solubility.

MeSH terms

Benzothiazoles
Fluorescent Dyes*
HeLa Cells
Humans
Hydrogen Sulfide*
Optical Imaging
Water

Substances

Benzothiazoles
Fluorescent Dyes
Water
Hydrogen Sulfide