NAD-seq for profiling the NAD + capped transcriptome of Arabidopsis thaliana

STAR Protoc. 2021 Nov 11;2(4):100901. doi: 10.1016/j.xpro.2021.100901. eCollection 2021 Dec 17.

Abstract

Eukaryotic RNAs can be modified with a non-canonical 5' nicotinamide adenine dinucleotide (NAD+) cap. NAD-seq identifies transcriptome-wide NAD+ capped RNAs. NAD-seq takes advantage of click chemistry to allow the capture of NAD+ capped RNAs. Unlike other approaches, NAD-seq does not require DNA synthesis on beads, but this technique uses full NAD+ capped transcripts eluted from beads as the substrates for strand-specific RNA sequencing library preparation. For complete details on the use and execution of this protocol, please refer to Yu et al. (2021).

Keywords: Gene Expression; Genomics; Model Organisms; Molecular Biology; Molecular/Chemical Probes; Plant sciences; RNAseq.