Genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers

STAR Protoc. 2021 Nov 10;2(4):100939. doi: 10.1016/j.xpro.2021.100939. eCollection 2021 Dec 17.

Abstract

Mosaic analysis with double markers (MADM) technology enables the generation of genetic mosaic tissue in mice. MADM enables concomitant fluorescent cell labeling and introduction of a mutation of a gene of interest with single-cell resolution. This protocol highlights major steps for the generation of genetic mosaic tissue and the isolation and processing of respective tissues for downstream histological analysis. For complete details on the use and execution of this protocol, please refer to Contreras et al. (2021).

Keywords: Developmental biology; Genetics; Microscopy; Model Organisms; Molecular Biology; Neuroscience; Single Cell; Stem Cells; Tissue Engineering.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Lineage / genetics
  • Female
  • Genetic Markers / genetics*
  • Genetic Techniques*
  • Histocytochemistry
  • Image Processing, Computer-Assisted
  • Male
  • Mice
  • Microscopy, Confocal
  • Mosaicism*
  • Single-Cell Analysis

Substances

  • Genetic Markers