Isolation of myeloid cells from mouse brain tumors for single-cell RNA-seq analysis

STAR Protoc. 2021 Nov 17;2(4):100957. doi: 10.1016/j.xpro.2021.100957. eCollection 2021 Dec 17.

Abstract

Current single-cell RNA sequencing (scRNA-seq) protocols are limited by the number of cells that can be simultaneously sequenced, restricting the ability to resolve heterogeneity of rare cell types. We describe here a protocol for rapid isolation of myeloid cells from tumor-harboring mouse cerebellum without cell sorting to minimize cell damage for scRNA-seq. This protocol includes the procedures for further enrichment of myeloid cells using CD11b+ magnetic beads, followed by the generation of scRNA library and sequencing analysis. For complete details on the use and execution of this protocol, please refer to Dang et al. (2021).

Keywords: Cancer; Cell Biology; Cell isolation; Immunology; RNAseq; Sequencing; Single Cell.