Direct evidence for chromosomal inversion during T-cell receptor beta-gene rearrangements

Nature. 1986 Jan 2-8;319(6048):28-33. doi: 10.1038/319028a0.


A germline T-cell receptor variable region (V beta) gene segment (V beta 14) has been mapped 10 kilobases to the 3' side of the constant region (C beta 2) gene. The V beta 14 gene segment is in an inverted transcriptional polarity relative to the diversity-region (D beta) and joining-region (J beta) gene segments and the C beta genes. Analyses of a T-cell clone (J 6.19), which has productively rearranged the V beta 14 gene segment, indicate that the productive V beta-D beta-J beta rearrangement and its reciprocal flank recombination product are linked and located at either border of a chromosomal inversion. These data demonstrate for the first time a linkage between mammalian V and C genes and verify that a functional T-cell receptor V beta gene can be constructed through a chromosomal inversion.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Cell Differentiation
  • Chromosome Inversion
  • Genes
  • Germ Cells / physiology
  • Humans
  • Receptors, Antigen, T-Cell / genetics*
  • Recombination, Genetic
  • T-Lymphocytes / physiology


  • Receptors, Antigen, T-Cell

Associated data

  • GENBANK/X03277
  • GENBANK/X03279