Androgen receptors in ventral prostate glands of zinc deficient rats

Life Sci. 1986 Jan 27;38(4):351-6. doi: 10.1016/0024-3205(86)90082-2.

Abstract

Androgen binding has been studied in the prostate cytosol of zinc deficient rats by charcoal assays. Rats were housed individually in plastic cages and maintained on a zinc deficient diet for 3 months. The cytosol fraction of prostate gland was incubated with various concentrations of tritiated methyltrienolone (3H-R1881, a synthetic androgen) alone or in the presence of 500-fold excess of radioinert R1881. Scatchard analysis of the data revealed that the number of androgen binding sites in the cytosol fraction of the zinc deficient rat prostate was 31 +/- 5.2 fmol/mg cytosol protein. This was significantly lower than that (84 +/- 11.5 fmol/mg protein) of the controls. Their dissociation constant (Kd = 1.6 +/- 0.6 nM) on the other hand was not different from that (1.7 +/- 0.7 nM) of control animals. This decrease in the concentration of cytosol receptor sites in the zinc deficient state suggests that this metal is involved in the androgen-binding process in the target cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Androstane-3,17-diol / metabolism
  • Animals
  • Cytosol / metabolism
  • Dihydrotestosterone / metabolism
  • Estrenes / metabolism
  • Kinetics
  • Male
  • Metribolone
  • Prostate / metabolism*
  • Rats
  • Receptors, Androgen / metabolism*
  • Testosterone / metabolism
  • Zinc / blood
  • Zinc / deficiency*

Substances

  • Estrenes
  • Receptors, Androgen
  • Dihydrotestosterone
  • Androstane-3,17-diol
  • Metribolone
  • Testosterone
  • Zinc