Phytochemical Analysis, In Vitro Antioxidant, and Wound Healing Activities of Turbinaria ornata (Turner) J. Agardh from Gulf of Mannar, India

Appl Biochem Biotechnol. 2022 Jan;194(1):395-406. doi: 10.1007/s12010-021-03752-0. Epub 2021 Dec 1.

Abstract

Turbinaria ornata (Turner) J., tropical brown algae, was found in the South Pacific and Indian Ocean ecosystems. In accordance with recent studies, Turbinaria ornata J. has potent anti-inflammatory effects. Therefore, this study is aimed to explore the biological activities of ethanolic extract of T. ornata J. by analyzing the presence of phytochemical components, antioxidant property, antimicrobial activity, and the wound healing activity. From the results, phytochemical analysis of ethanolic extract of T. ornata J. showed the presence of alkaloids, saponins, oils, total phenolic, and total flavonoid content which were estimated to be 0.683±0.001 Abs and 0.433±0.001 Abs, respectively. Antioxidant activity of the ethanolic extract of T. ornata J. extract showed remarkable DPPH radical scavenging activity of about 58.8% at 200μg/mL and total antioxidant activity of 0.257 Abs at 100μg/mL concentration, as compared to that of their respective controls. The ethanolic extract of T. ornata J. exhibited the maximum zone of inhibition against the clinical pathogens like Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Candida albicans, and Methicillin-resistant Staphylococcus aureus with their potent antimicrobial activity. Wound healing effects of the ethanolic extract of T. ornata J were analyzed by using zebrafish model. The results showed the rapid and significant regeneration of the wounded caudal fin on day 14. Therefore, the preliminary results of this study strongly support that the ethanolic extract of T. ornata J. may be effective in wound healing and regeneration of the wounded tissues.

Keywords: Antimicrobial; Antioxidant; Turbinaria ornata J.; Wound healing; Zebrafish.