De-Insertion Current Analysis of Pore-Forming Peptides and Proteins Using Gold Electrode-Supported Lipid Bilayer

Kan Shoji

doi:10.1007/978-1-0716-1843-1_8

De-Insertion Current Analysis of Pore-Forming Peptides and Proteins Using Gold Electrode-Supported Lipid Bilayer

Methods Mol Biol. 2022:2402:93-102. doi: 10.1007/978-1-0716-1843-1_8.

Author

Kan Shoji¹

Affiliation

¹ Department of Mechanical Engineering, Nagaoka University of Technology, Niigata, Japan. kshoji@mech.nagaokaut.ac.jp.

PMID: 34854038
DOI: 10.1007/978-1-0716-1843-1_8

Abstract

Analysis of the pore formation mechanisms of transmembrane peptides and proteins potentially provides an insight into cellular biology because of their significant roles in biological systems. Here, we describe the procedures to analyze the pore formation mechanisms of transmembrane peptides and proteins by measuring de-insertion currents of biological nanopores that can be obtained by unzipping the lipid bilayers.

Keywords: Channel current measurements; De-insertion current analysis; Gold needle; Pore formation mechanism; Supported bilayer lipid membranes; Transmembrane peptides/proteins.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Electrodes
Gold
Lipid Bilayers*
Peptides
Proteins

Substances

Lipid Bilayers
Peptides
Proteins
Gold