Selective inhibition of lipopolysaccharide-induced polyclonal IgG response by monoclonal Ly-5 antibody

J Immunol. 1986 Apr 15;136(8):2729-33.

Abstract

We have identified two important molecules involved in the regulation of B cell differentiation, namely Lyb-2 and Ly-5. To gain further insight into the function of these two molecules, we examined the effect of monoclonal Lyb-2 and Ly-5 antibodies on lipopolysaccharide (LPS)-induced B cell growth and maturation. We found that Lyb-2 antibody does not have any effect on LPS-induced proliferation and on polyclonal IgM or total IgG responses. On the other hand, although Ly-5 antibody did not affect proliferation and polyclonal IgM responses, it strongly inhibited polyclonal IgG responses, presumably by direct action on B cells. This inhibition was not caused by direct suppressive effect of Ly-5 antibody or Fc receptor-mediated negative signaling. To exert maximal inhibitory effect, Ly-5 antibody had to be added to the culture during the initial 48 hr. However, the presence of Ly-5 antibody during the first 2 days did not cause a significant inhibition. It is thus likely that Ly-5 plays a critical role in the regulation of LPS-induced B cell maturation into IgG-secreting cells at a phase starting within 48 hr after LPS stimulation and continuing thereafter.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / physiology*
  • Antigens, Ly / immunology*
  • B-Lymphocytes / cytology
  • B-Lymphocytes / immunology
  • Cell Cycle
  • Cell Differentiation
  • Cell Separation
  • Immune Tolerance*
  • Immunoglobulin G / biosynthesis*
  • Lipopolysaccharides / pharmacology*
  • Lymphocyte Activation
  • Macrophages / immunology
  • Mice
  • Mice, Inbred Strains
  • Spleen
  • T-Lymphocytes / immunology

Substances

  • Antibodies, Monoclonal
  • Antigens, Ly
  • Immunoglobulin G
  • Lipopolysaccharides