Gut injury in mouse graft-versus-host reaction. Study of its occurrence and mechanisms

J Clin Invest. 1986 May;77(5):1584-95. doi: 10.1172/JCI112474.

Abstract

The occurrence, nature, and pathogenesis of intestinal lesions were studied in a number of graft vs. host reaction (GVHR) conditions in mice, combining variations in the nature of the following: the F1 hosts (newborn or adult, normal or lethally irradiated), the injected parental T cells (mixed or selected subsets of Lyt2+ or L3T4+ cells), and the antigenic stimulus (semi-allogeneic or restricted to class I or II MHC loci). The following conclusions were drawn: Three gut alterations are always associated: donor T cell infiltration, predominating in the crypt region; acceleration of the epithelium renewal; and increased epithelial Ia expression. The initial event is T-cell infiltration, which results from stimulation within the Peyer patches followed by cyclic traffic, i.e., migration into the thoracic duct and then seeding to the whole gut mucosa. Both Lyt2+ and L3T4+ cells can infiltrate the gut wall, the extent of the infiltration by a given subset depending upon the capacity of the donor blasts to circulate in the thoracic duct (higher for L3T4+) and then to home in the gut (much higher for Lyt2+ blasts) and the nature of the alloantigenic stimulation that governs the extent of each donor subset proliferation. Both donor T-cell subsets can induce gut epithelial damage, but for a comparable amount of infiltrating cells, L3T4+ cells induce more lesions. When the antigenic stimulation is restricted to class I or class I MHC loci, gut GVHR is much more easily elicited across class II MHC differences, which stimulate preferentially L3T4+ donor cells. The main mechanism of epithelial damage is not direct cytotoxicity, but more probably lymphokine(s) release.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Epithelium / pathology
  • Graft vs Host Reaction*
  • HLA Antigens / immunology
  • Histocompatibility Antigens Class II / immunology
  • Intestinal Mucosa / pathology
  • Intestines / pathology*
  • Lymphokines / metabolism
  • Major Histocompatibility Complex
  • Mice
  • Mice, Inbred C3H
  • Mice, Inbred DBA
  • T-Lymphocytes / classification
  • T-Lymphocytes / pathology
  • Thoracic Duct / pathology

Substances

  • HLA Antigens
  • Histocompatibility Antigens Class II
  • Lymphokines