Formalin-fixed paraffin-embedded tissues for microbiome analysis in rainbow trout (Oncorhynchus mykiss)

J Microbiol Methods. 2022 Jan:192:106389. doi: 10.1016/j.mimet.2021.106389. Epub 2021 Dec 2.

Abstract

The gut microbiomes of rainbow trout (Oncorhynchus mykiss) reared at 16° and 22 °C were determined using formalin-fixed paraffin-embedded tissues (FFPE) and compared to fresh frozen tissue. The data revealed microbiomes could be successfully determined using FFPE tissue opening a new horizon in studying intestinal microbiota using archived histological samples.

Keywords: Formalin-fixed paraffin-embedded; Microbiome; Rainbow trout; Temperature.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cryopreservation*
  • Fixatives / pharmacology*
  • Formaldehyde / pharmacology*
  • Gastrointestinal Microbiome / genetics*
  • High-Throughput Nucleotide Sequencing
  • Oncorhynchus mykiss / microbiology*
  • Paraffin Embedding*
  • RNA, Ribosomal, 16S / genetics
  • Temperature
  • Tissue Fixation

Substances

  • Fixatives
  • RNA, Ribosomal, 16S
  • Formaldehyde