Complementation of fission yeast kinesin-5/Cut7 with human Eg5 provides a versatile platform for screening of anti-cancer compounds

Biosci Biotechnol Biochem. 2021 Dec 4;zbab212. doi: 10.1093/bbb/zbab212. Online ahead of print.

Abstract

Kinesin-5 family proteins are essential for bipolar spindle assembly to ensure mitotic fidelity. Here we demonstrate evolutionary functional conservation of kinesin-5 between human and fission yeast. Human Eg5 expressed in the nucleus replaces fission yeast counterpart Cut7. Intriguingly, Eg5 overproduction results in cytotoxicity. This phenotype provides a useful platform for the development of novel kinesin-5 inhibitors as anti-cancer drugs.

Keywords: Cut7; Eg5; fission yeast; kinesin-5; mitotic spindle.