Complementation of fission yeast kinesin-5/Cut7 with human Eg5 provides a versatile platform for screening of anticancer compounds

Biosci Biotechnol Biochem. 2022 Jan 24;86(2):254-259. doi: 10.1093/bbb/zbab212.


Kinesin-5 family proteins are essential for bipolar spindle assembly to ensure mitotic fidelity. Here, we demonstrate evolutionary functional conservation of kinesin-5 between human and fission yeast. Human Eg5 expressed in the nucleus replaces fission yeast counterpart Cut7. Intriguingly, Eg5 overproduction results in cytotoxicity. This phenotype provides a useful platform for the development of novel kinesin-5 inhibitors as anticancer drugs.

Keywords: Cut7; Eg5; fission yeast; kinesin-5; mitotic spindle.

MeSH terms

  • Schizosaccharomyces*