Evaluating microglial phenotypes using single-cell technologies

Trends Neurosci. 2022 Feb;45(2):133-144. doi: 10.1016/j.tins.2021.11.001. Epub 2021 Dec 4.


Recent single-cell technologies have enabled researchers to simultaneously assess the transcriptomes and other modalities of thousands of cells within their spatial context. Here, we have summarized available single-cell methods for dissociated tissues and tissue slides with respect to the specifics of microglial biology. We have focused on next-generation-based technologies. We review the potential of these single-cell sequencing methods and newer multiomics approaches to extend the understanding of microglia function beyond the status quo.

Keywords: immunity; microglia; multiomics; single-cell sequencing; spatial context.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Humans
  • Microglia*
  • Phenotype
  • Transcriptome*