A genetically encoded fluorescent biosensor for extracellular L-lactate

Yusuke Nasu; Ciaran Murphy-Royal; Yurong Wen; Jordan N Haidey; Rosana S Molina; Abhi Aggarwal; Shuce Zhang; Yuki Kamijo; Marie-Eve Paquet; Kaspar Podgorski; Mikhail Drobizhev; Jaideep S Bains; M Joanne Lemieux; Grant R Gordon; Robert E Campbell

doi:10.1038/s41467-021-27332-2

A genetically encoded fluorescent biosensor for extracellular L-lactate

Nat Commun. 2021 Dec 6;12(1):7058. doi: 10.1038/s41467-021-27332-2.

Authors

Yusuke Nasu¹, Ciaran Murphy-Royal^{2

3}, Yurong Wen^{4

5}, Jordan N Haidey², Rosana S Molina⁶, Abhi Aggarwal⁷, Shuce Zhang⁸, Yuki Kamijo¹, Marie-Eve Paquet^{9

10}, Kaspar Podgorski⁷, Mikhail Drobizhev⁶, Jaideep S Bains², M Joanne Lemieux⁴, Grant R Gordon², Robert E Campbell^{11

12}

Affiliations

¹ Department of Chemistry, School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, 113-0033, Japan.
² Hotchkiss Brain Institute, Cumming School of Medicine, Department of Physiology and Pharmacology, University of Calgary, Calgary, AB, T2N 4N1, Canada.
³ Centre Hospitalier de l'Université de Montréal, Department of Neuroscience, Faculty of Medicine, University of Montreal, Montreal, QC, H2X 0A9, Canada.
⁴ Department of Biochemistry, University of Alberta, Edmonton, AB, T6G 2H7, Canada.
⁵ Department of Talent Highland, The First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, China.
⁶ Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT, 59717, USA.
⁷ Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, 20147, USA.
⁸ Department of Chemistry, University of Alberta, Edmonton, AB, T6G 2G2, Canada.
⁹ CERVO Brain Research Center, Laval University, Québec, QC, G1E 1T2, Canada.
¹⁰ Department of Biochemistry, Microbiology and Bioinformatics, Laval University, Québec, QC, G1J 2G3, Canada.
¹¹ Department of Chemistry, School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, 113-0033, Japan. campbell@chem.s.u-tokyo.ac.jp.
¹² Department of Chemistry, University of Alberta, Edmonton, AB, T6G 2G2, Canada. campbell@chem.s.u-tokyo.ac.jp.

Abstract

L-Lactate, traditionally considered a metabolic waste product, is increasingly recognized as an important intercellular energy currency in mammals. To enable investigations of the emerging roles of intercellular shuttling of L-lactate, we now report an intensiometric green fluorescent genetically encoded biosensor for extracellular L-lactate. This biosensor, designated eLACCO1.1, enables cellular resolution imaging of extracellular L-lactate in cultured mammalian cells and brain tissue.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / genetics
Bacterial Proteins / metabolism*
Binding Sites / genetics
Biosensing Techniques / methods*
Cell Line, Tumor
Crystallography, X-Ray
Fluorescence
Green Fluorescent Proteins / chemistry
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism*
HEK293 Cells
HeLa Cells
Humans
Lactic Acid / analysis*
Lactic Acid / metabolism
Microscopy, Fluorescence
Periplasmic Proteins / genetics
Periplasmic Proteins / metabolism*
Protein Binding
Recombinant Fusion Proteins / chemistry
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism*
Reproducibility of Results

Substances

Bacterial Proteins
Periplasmic Proteins
Recombinant Fusion Proteins
Green Fluorescent Proteins
Lactic Acid

Abstract

Publication types

MeSH terms

Substances

Grants and funding