Structure of pathological TDP-43 filaments from ALS with FTLD

Nature. 2022 Jan;601(7891):139-143. doi: 10.1038/s41586-021-04199-3. Epub 2021 Dec 8.


The abnormal aggregation of TAR DNA-binding protein 43 kDa (TDP-43) in neurons and glia is the defining pathological hallmark of the neurodegenerative disease amyotrophic lateral sclerosis (ALS) and multiple forms of frontotemporal lobar degeneration (FTLD)1,2. It is also common in other diseases, including Alzheimer's and Parkinson's. No disease-modifying therapies exist for these conditions and early diagnosis is not possible. The structures of pathological TDP-43 aggregates are unknown. Here we used cryo-electron microscopy to determine the structures of aggregated TDP-43 in the frontal and motor cortices of an individual who had ALS with FTLD and from the frontal cortex of a second individual with the same diagnosis. An identical amyloid-like filament structure comprising a single protofilament was found in both brain regions and individuals. The ordered filament core spans residues 282-360 in the TDP-43 low-complexity domain and adopts a previously undescribed double-spiral-shaped fold, which shows no similarity to those of TDP-43 filaments formed in vitro3,4. An abundance of glycine and neutral polar residues facilitates numerous turns and restricts β-strand length, which results in an absence of β-sheet stacking that is associated with cross-β amyloid structure. An uneven distribution of residues gives rise to structurally and chemically distinct surfaces that face external densities and suggest possible ligand-binding sites. This work enhances our understanding of the molecular pathogenesis of ALS and FTLD and informs the development of diagnostic and therapeutic agents that target aggregated TDP-43.

Publication types

  • Case Reports

MeSH terms

  • Amino Acid Sequence
  • Amyloid beta-Peptides / metabolism
  • Amyotrophic Lateral Sclerosis / metabolism*
  • Amyotrophic Lateral Sclerosis / pathology*
  • Cryoelectron Microscopy*
  • DNA-Binding Proteins / chemistry*
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • DNA-Binding Proteins / ultrastructure*
  • Female
  • Frontal Lobe / metabolism
  • Frontal Lobe / pathology
  • Frontal Lobe / ultrastructure
  • Frontotemporal Lobar Degeneration / metabolism*
  • Frontotemporal Lobar Degeneration / pathology*
  • Humans
  • Male
  • Middle Aged
  • Motor Cortex / metabolism
  • Motor Cortex / pathology
  • Motor Cortex / ultrastructure
  • Mutation


  • Amyloid beta-Peptides
  • DNA-Binding Proteins
  • TARDBP protein, human