Role of Ly-6 in lymphocyte activation. II. Induction of T cell activation by monoclonal anti-Ly-6 antibodies

J Exp Med. 1986 Sep 1;164(3):709-22. doi: 10.1084/jem.164.3.709.


The Ly-6 locus controls the expression and/or encodes for alloantigenic specificities found primarily on subpopulations of murine T and B lymphocytes. We have recently identified and characterized a new rat mAb, D7, that recognizes a nonpolymorphic Ly-6 specificity. After crosslinking by anti-Ig reagents or by Fc receptor-bearing accessory cells, mAb D7 could induce IL-2 production from T cell hybridomas, and in the presence of PMA could trigger a vigorous proliferative response in resting peripheral T cells. The addition of mAb D7 to cultures of antigen- and alloantigen-, but not mitogen-stimulated T cells resulted in a marked augmentation of the proliferative response. A number of other well-characterized mAbs to Ly-6 locus products could also stimulate a T cell proliferative response after crosslinking by anti-Ig and in the presence of PMA. These results strongly suggest that Ly-6 molecules may play a critical role in the T cell activation cascade, either as receptors for an unidentified soluble or cell-associated ligand or as transducing molecules that modulate signals initiated by antigen stimulation of the T3-Ti complex.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Antigen-Presenting Cells / physiology
  • Antigens, Ly / immunology*
  • Calcium / metabolism
  • Hybridomas / metabolism
  • Interleukin-2 / metabolism
  • Lymphocyte Activation*
  • Mice
  • Mitogens / pharmacology
  • Receptors, Fc / physiology
  • T-Lymphocytes / immunology*
  • Tetradecanoylphorbol Acetate / pharmacology


  • Antibodies, Monoclonal
  • Antigens, Ly
  • Interleukin-2
  • Mitogens
  • Receptors, Fc
  • Tetradecanoylphorbol Acetate
  • Calcium