Modulation of bovine serum albumin aggregation by glutathione functionalized MoS2 quantum dots

Abstract

In present study, a novel glutathione functionalized MoS₂ quantum dots (GSH-MoS₂ QDs) was synthesized from sodium molybdate dehydrate and glutathione by using a one-pot hydrothermal method. After they were characterized, the influence of GSH-MoS₂ QDs on amyloid aggregation of bovine serum albumin (BSA) was investigated by various analytical methods including thioflavin T fluorescence assay, circular dichroism and transmission electron microscope. Moreover, the effect of GSH-MoS₂ QDs on cytotoxicity induced by BSA amyloid fibrils and cell penetration were evaluated by MTT assay and confocal fluorescence imaging, respectively. The results indicated that the GSH-MoS₂ QDs not only had good water solubility, excellent biocompatibility and low cytotoxicity, but also could obviously inhibit the aggregation of BSA and depolymerize the formed BSA aggregates. The data obtained from this work demonstrated that the GSH-MoS₂ QDs is expected to become a candidate drug for the treatment of amyloid-related diseases.

Keywords: Amyloid fibril; Bovine serum albumin; Cytotoxicity; Glutathione; MoS(2) quantum dots.