Protective effects of non-extractable phenolics from strawberry against inflammation and colon cancer in vitro

Meigui Huang; Yanhui Han; Lingfei Li; Kanyasiri Rakariyatham; Xian Wu; Zili Gao; Hang Xiao

doi:10.1016/j.foodchem.2021.131759

Protective effects of non-extractable phenolics from strawberry against inflammation and colon cancer in vitro

Food Chem. 2022 Apr 16:374:131759. doi: 10.1016/j.foodchem.2021.131759. Epub 2021 Dec 2.

Authors

Meigui Huang¹, Yanhui Han², Lingfei Li³, Kanyasiri Rakariyatham², Xian Wu⁴, Zili Gao², Hang Xiao⁵

Affiliations

¹ Department of Food Science and Engineering, College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing, Jiangsu 210037, PR China; Department of Food Science, University of Massachusetts, Amherst, MA 01003, United States.
² Department of Food Science, University of Massachusetts, Amherst, MA 01003, United States.
³ Department of Food Science, University of Massachusetts, Amherst, MA 01003, United States; College of Food Science and Technology, Yunnan Agricultural University, Kunming, Yunnan 650000, PR China.
⁴ Department of Food Science, University of Massachusetts, Amherst, MA 01003, United States; Department of Kinesiology and Health, Miami University, Oxford, OH 45056, USA.
⁵ Department of Food Science, University of Massachusetts, Amherst, MA 01003, United States. Electronic address: hangxiao@foodsci.umass.edu.

PMID: 34896944
DOI: 10.1016/j.foodchem.2021.131759

Abstract

Strawberry is a rich source of phenolics. However, most studies focused on extractable phenolics (EP) while neglecting non-extractable phenolics (NEP). The aim of this study was to characterize EP and NEP from strawberry (Fragaria × ananassa) and determine their anti-inflammatory and anti-colon cancer potentials in cell culture models. NEP contained flavonols, flavanols and phenolic acids that were released through alkaline hydrolysis. NEP dose-dependently inhibited lipopolysaccharides -induced NO production in RAW 264.7 macrophage. Western blotting showed that NEP reduced the expression levels of pro-inflammatory proteins such as iNOS and c-FOS, but increased the expression level of antioxidative protein, such as HO-1. Moreover, NEP markedly suppressed proliferation of human colon cancer HCT116 cells via inducing G2/M phase cell cycle arrest and apoptosis. Collectively, these findings illustrated preventive effects of strawberry NEP against inflammation and colon cancer, shedding light on potential contribution of NEP from strawberry as a health-promoting agent.

Keywords: Anti-cancer; Anti-inflammation; Non-extractable phenolics; Strawberry.

MeSH terms

Colonic Neoplasms*
Fragaria*
Fruit / chemistry
Humans
Inflammation
Phenols / analysis
Polyphenols

Substances

Phenols
Polyphenols