Review of ovarian tissue cryopreservation techniques for fertility preservation

J Gynecol Obstet Hum Reprod. 2022 Feb;51(2):102290. doi: 10.1016/j.jogoh.2021.102290. Epub 2021 Dec 11.


Ovarian failure and ovarian malfunction are among major fertility problems in women of reproductive age (18-35 years). It is known that various diseases, such as ovarian cancer and premature ovarian failure, besides certain treatments, such as radiotherapy and chemotherapy of other organs, can affect the normal process of folliculogenesis and cause infertility. In recent years, various procedures have been proposed for the treatment of infertility. One of the newest methods is the use of cryopreservation ovarian fragments after cancer treatment. According to some studies, this method yields very satisfactory results. Although ovarian tissue cryopreservation (OTC) is an accepted technique of fertility preservation, the relative efficacy of cryopreservation protocols remains controversial. Considering the controversies about these methods and their results, in this study, we aimed to compare different techniques of ovarian cryopreservation and investigate their advantages and disadvantages. Reviewing the published articles may be possible to identify appropriate strategies and improve infertility treatment in these patients.

Keywords: Fertility; Ovarian tissue cryopreservation; Slow-freezing; Vitrification.

Publication types

  • Comparative Study
  • Review

MeSH terms

  • Cryopreservation / methods*
  • Female
  • Fertility Preservation / methods*
  • Humans