Ecotoxicological evaluation of zebrafish liver (Danio rerio) induced by dibutyl phthalate

Abstract

Dibutyl phthalate (DBP), one of the most commonly applied plasticizers, has been frequently detected in the aquatic environment, posing potential risks to aquatic organisms. Currently, reports about the toxicity of zebrafish liver with DBP exposure are rare, and the toxic mechanism is still not clear. In this study, zebrafish (Danio rerio) were used to explore the ecotoxicological effects of DBP from the physiological, biochemical, genetic, and molecular levels. The results showed oxidative stress, lipid peroxidation, and DNA damage occurred in zebrafish liver according to changes in antioxidant enzymes, MDA and 8-OHdG content. AchE activity was always active, and negatively correlated with the DBP concentration. The expression of Cu/Zn-sod and gpx genes were similar to that of antioxidant enzymes from 7 to 21 days, while in the end, the inconsistent result appeared due to the time lag effect in protein modification, gene transcription and translation. Besides, the mRNA abundance of Caspase-3 and p53 were upregulated, showing a "dose-response" relationship. The integrated biomarker reaction indicated that the effects of exposure time on zebrafish liver was 14th day> 28th day> 7th day> 21th day. These results are of great significance to evaluate the toxicological effects and explore the toxic mechanism of DBP on aquatic organisms.

Keywords: Antioxidant and apoptosis genes; Dibutyl phthalate (DBP); Neurotoxicity; Oxidative and DNA damage; Zebrafish.