Culturing Human Pluripotent Stem Cells on Micropatterned Silicon Surfaces

Methods Mol Biol. 2022;2454:49-59. doi: 10.1007/7651_2021_428.

Abstract

Human pluripotent stem cell culture conditions are constantly being optimized, thus providing insight to the environmental cues that affect cell choices. A wide variety of media, coating materials, and substrates is now available for use, serving different scientific needs. Factors such as material stiffness, roughness, and topography are being recognized to contribute or even direct the acquisition of specific phenotypes. Here, we describe the use of patterned silicon substrates coated with Matrigel for the propagation and differentiation of human pluripotent stem cells.

Keywords: BRACHYURY; Immunofluorescence; Laser manufacturing; NANOG; Silicon substrates; Surface micropatterning; Topography; hPSCs.

MeSH terms

  • Cell Differentiation / genetics
  • Humans
  • Pluripotent Stem Cells*
  • Silicon*

Substances

  • Silicon