Piezo1 controls cell volume and migration by modulating swelling-activated chloride current through Ca2+ influx

Abstract

Regulatory volume decrease (RVD), a homeostatic process responsible for the re-establishment of the original cell volume upon swelling, is critical in controlling several functions, including migration. RVD is mainly sustained by the swelling-activated Cl^- current (I_Cl,swell ), which can be modulated by cytoplasmic Ca²⁺ . Cell swelling also activates mechanosensitive channels, including the ubiquitously expressed Ca²⁺ -permeable channel Piezo1. We hypothesized that, by controlling cytoplasmic Ca²⁺ and in turn I_Cl,swell , Piezo1 is involved in the fine regulation of RVD and cell migration. We compared RVD and I_Cl,swell in wild-type (WT) HEK293T cells, which express endogenous levels of Piezo1, and in cells overexpressing (OVER) or knockout (KO) for Piezo1. Compared to WT, RVD was markedly increased in OVER, while virtually absent in KO cells. Consistently, I_Cl,swell amplitude was highest in OVER and lowest in KO cells, with WT cells displaying an intermediate level, suggesting a Ca²⁺ -dependent modulation of the current by Piezo1 channels. Indeed, in the absence of external Ca²⁺ , I_Cl,swell in both WT and OVER cells, as well as the RVD probed in OVER cells, were significantly lower than in the presence of Ca²⁺ and no longer different compared to KO cells. However, the Piezo-mediated Ca²⁺ influx was ineffective in enhancing I_Cl,swell in the absence of releasable Ca²⁺ from intracellular stores. The different expression levels of Piezo1 affected also cell migration which was strongly enhanced in OVER, while reduced in KO cells, as compared to WT. Taken together, our data indicate that Piezo1 controls RVD and migration in HEK293T cells by modulating I_Cl,swell through Ca²⁺ influx.

Keywords: Ca2+ entry; ICl,swell; Piezo1; cell migration; regulatory volume decrease.