Complementary DNA for a novel human interleukin (BSF-2) that induces B lymphocytes to produce immunoglobulin

Nature. 1986 Nov 6-12;324(6092):73-6. doi: 10.1038/324073a0.

Abstract

When stimulated with antigen, B cells are influenced by T cells to proliferate and differentiate into antibody-forming cells. Since it was reported that soluble factors could replace certain functions of helper T cells in the antibody response, several different kinds of lymphokines and monokines have been reported in B-cell growth and differentiation. Among these, human B-cell differentiation factor (BCDF or BSF-2) has been shown to induce the final maturation of B cells into immunoglobulin-secreting cells. BSF-2 was purified to homogeneity and its partial NH2-terminal amino-acid sequence was determined. These studies indicated that BSF-2 is functionally and structurally unlike other known proteins. Here, we report the molecular cloning, structural analysis and functional expression of the cDNA encoding human BSF-2. The primary sequence of BSF-2 deduced from the cDNA reveals that BSF-2 is a novel interleukin consisting of 184 amino acids.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antibody-Producing Cells / immunology*
  • B-Lymphocytes / immunology*
  • Base Sequence
  • Cell Differentiation
  • DNA / metabolism
  • Growth Substances / analysis*
  • Humans
  • Immunoglobulins / metabolism
  • Interleukin-4
  • Lymphokines / analysis*

Substances

  • Growth Substances
  • Immunoglobulins
  • Lymphokines
  • Interleukin-4
  • DNA

Associated data

  • GENBANK/X04602