Improving soil fertility by driving microbial community changes in saline soils of Yellow River Delta under petroleum pollution

Abstract

It is promising to use indigenous microorganisms for fertility improvement in petroleum-contaminated coastal soil. As a result, the microbial community and physicochemical property are the base for the restoration. For the detailed information, the Phragmites Communis (P), Chinese Tamarisk (C), Suaeda salsa (S), and new Bare Land (B) soil of Yellow River Delta was 90 g in 100 mL sterile bottles simulated at 25 °C with soil: petroleum = 10:1 in the incubator for four months. The samples were detected at 60 and 120 days along with untreated soil and aged Oil Sludge (O) as control. The results showed that all the samples were alkaline (pH 7.99-8.83), which the salinity and NO₃^- content of incubate soil followed the in situ samples as P (1.09-1.72‰, 8.02-8.17 mg kg^-1), C (10.61-13.79‰, 5.99-6.07 mg kg^-1), S (10.19-12.43‰, 3.64-4.22 mg kg^-1), B (31.85-32.45‰, 3.56-3.72 mg kg^-1) and O (31.61-34.30‰, 0.89-0.90 mg kg^-1). NO₃^- and organic carbon decreased after incubation, which the polluted samples (86.63-92.63 g kg^-1) still had higher organic carbon than untreated ones with more NH₄⁺ consumption. The high-throughput sequence results showed that the Gammaproteobacteria and Alphaproteobacteria were dominant in all samples, while sulfate reducting bacteria Alphaproteobacteria decreased at 120 days. Meanwhile, the electroactive Gammaproteobacteria might symbiosis with Methanosaetaceae and Methanosarcinaceae, degrading petroleum after electron receptors depletion. Nitrososphaeraceae and Nitrosopumilaceae oxidise NH₄⁺ to NO₂^- for intra-aerobic anaerobes and denitrifying bacteria producing oxygen for biodegradation in polluted Phragmites Communis soil. The halotolerant Halomicrobiaceae and Haloferacaceae predominated in saline Chinese Tamarisk, Suaeda Salsa and Bare Land, which were potential electroactive degradater. As the ageing sludge formed, the hydrogen trophic methanogens Methanothermobacteraceae (73.90-92.72%) was prevalent with the petroleum pollution. In conclusion, petroleum initiated two-phase in the sludge forming progress: electron acceptor consumption and electron transfer between degradater and methanogens. Based on the results, the domestic sewage N, P removal coupling and electron transport will be the basement for polluted soils fertility improvement.

Keywords: High-throughput sequence; Microbial community; Petroleum pollution; Physicochemical property; Yellow river delta.