Generation of mixed murine organoids to model cellular interactions

Ana Krotenberg Garcia; Jacco van Rheenen; Saskia Jacoba Elisabeth Suijkerbuijk

doi:10.1016/j.xpro.2021.100997

Generation of mixed murine organoids to model cellular interactions

STAR Protoc. 2021 Dec 8;2(4):100997. doi: 10.1016/j.xpro.2021.100997. eCollection 2021 Dec 17.

Authors

Ana Krotenberg Garcia¹, Jacco van Rheenen¹, Saskia Jacoba Elisabeth Suijkerbuijk¹

Affiliation

¹ Department of Molecular Pathology, Oncode Institute, Netherlands Cancer Institute, Amsterdam 1066 CX, The Netherlands.

Abstract

Cell competition is a mechanism of interaction that dictates cell selection based on differences in cellular fitness. We designed a protocol to generate mixed murine organoids and enteroid monolayers used to study such complex cellular interactions in a mammalian system. This protocol is dedicated to follow the behavior of different cell populations over time, using (time-lapse) microscopy or transcriptome/proteome analysis. For complete details on the use and execution of this protocol, please refer to Krotenberg Garcia et al. (2021).

Keywords: Cancer; Cell Biology; Cell culture; Microscopy; Organoids.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Communication / physiology*
Cell Culture Techniques
Cells, Cultured
Mice
Organoids / cytology*
Tissue Culture Techniques / methods*