A macrophage-T cell coculture model for severe tissue injury-induced T cell death

STAR Protoc. 2021 Dec 1;2(4):100983. doi: 10.1016/j.xpro.2021.100983. eCollection 2021 Dec 17.


A key observation of tissue injury, such as stroke and burn, is a state of systemic immunosuppression characterized by loss of T cells and rise of infections. Here, we present an in vitro model for cell-cell interactions between innate (macrophages) and adaptive (T cells) immune cells. This protocol facilitates bone marrow-derived macrophages (BMDMs) and splenic T cells in a coculture model. The procedure mimics injury-induced T cell death, which is driven by inflammasome activation in macrophages. For complete details on the use and execution of this protocol, please refer to Roth et al. (2021).

Keywords: Cell Biology; Cell culture; Cell isolation; Health Sciences; Immunology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Death / physiology*
  • Coculture Techniques / methods*
  • Female
  • Inflammasomes / immunology
  • Inflammasomes / metabolism
  • Macrophages / cytology*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Models, Immunological*
  • Spleen / cytology
  • T-Lymphocytes / cytology*
  • Wounds and Injuries / immunology
  • Wounds and Injuries / metabolism


  • Inflammasomes