The effect of Gla-containing proteins on the precipitation of insoluble salts

Biochem Biophys Res Commun. 1987 Jan 15;142(1):113-9. doi: 10.1016/0006-291x(87)90458-x.


The precipitation of insoluble salts containing divalent metal ions is inhibited by Gla-containing proteins of various origin. In this paper we demonstrate that: Gla-residues are required for the inhibitory activity; the inhibition is effected by a protein which in vivo is bound to calcified tissue (osteocalcin) as well as by proteins occurring in blood plasma (factor X) and urine (the urinary Gla-protein); The inhibitor concentration required for 50% precipitation-inhibition varied slightly from one salt to the other, but no marked differences were observed between the effects of the various Gla-containing proteins used; Precipitation-inhibition occurred in all phosphates (Be, Ca, Mn and Zn) and in all calcium salts (phosphate, oxalate and carbonate) tested.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 1-Carboxyglutamic Acid / physiology*
  • Calcium Phosphates / metabolism
  • Calcium-Binding Proteins / metabolism*
  • Cations, Divalent / metabolism
  • Chemical Precipitation
  • Factor X / metabolism
  • Osteocalcin
  • Phosphates / metabolism
  • Salts / metabolism*
  • Solubility
  • Vitamin K / physiology


  • Calcium Phosphates
  • Calcium-Binding Proteins
  • Cations, Divalent
  • Phosphates
  • Salts
  • alpha-tricalcium phosphate
  • tetracalcium phosphate
  • Osteocalcin
  • Vitamin K
  • 1-Carboxyglutamic Acid
  • calcium phosphate, monobasic, anhydrous
  • Factor X
  • calcium phosphate
  • calcium phosphate, dibasic, anhydrous