High efficiency antigen presentation by thyroglobulin-primed murine splenic B cells

Eur J Immunol. 1987 Mar;17(3):393-8. doi: 10.1002/eji.1830170314.

Abstract

B cells primed in vivo with mouse or rat thyroglobulin present these antigens at very low concentrations to CH9, an Ly 1+2- T cell hybridoma specific for mouse and rat thyroglobulin. Presentation measured by interleukin 2 release from CH9 is sensitive to treatment with a monoclonal antibody eliminating splenic B cells but is unaffected by anti-Thy-1.2 or 33D1 (which destroy T cells and dendritic cells, respectively). Presentation is specific for the priming antigen and is blocked by preincubation of the B cells with sheep anti-mouse F(ab')2. We suggest that in this system, primed B cells present thyroglobulin and that this may represent a means by which an initial triggering event priming both B and T cells could allow maintenance of autoreactive responses in vivo in the presence of low concentrations of circulating antigen.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigen-Presenting Cells / immunology*
  • Autoimmune Diseases / immunology*
  • B-Lymphocytes / immunology*
  • Cell Line
  • Dendritic Cells / physiology
  • Immunoglobulin Fab Fragments / immunology
  • Lymphocyte Activation
  • Mice
  • T-Lymphocytes / immunology*
  • Thyroglobulin / immunology*

Substances

  • Immunoglobulin Fab Fragments
  • Thyroglobulin