Dietary Amylose/Amylopectin Ratio Modulates Cecal Microbiota and Metabolites in Weaned Goats

Kefyalew Gebeyew; Kai Chen; Teketay Wassie; Md Abul Kalam Azad; Jianhua He; Weimin Jiang; Wu Song; Zhixiong He; Zhiliang Tan

doi:10.3389/fnut.2021.774766

Dietary Amylose/Amylopectin Ratio Modulates Cecal Microbiota and Metabolites in Weaned Goats

Front Nutr. 2021 Nov 23:8:774766. doi: 10.3389/fnut.2021.774766. eCollection 2021.

Authors

Kefyalew Gebeyew^{1

2}, Kai Chen^{1

3}, Teketay Wassie¹, Md Abul Kalam Azad¹, Jianhua He³, Weimin Jiang⁴, Wu Song⁴, Zhixiong He^{1

2}, Zhiliang Tan^{1

2}

Affiliations

¹ CAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Processes, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, China.
² University of Chinese Academy of Sciences, Beijing, China.
³ College of Animal Science and Technology, Hunan Agricultural University, Changsha, China.
⁴ Herbivore Nutrition Department, Hunan Institute of Animal and Veterinary Science, Changsha, China.

Abstract

Increasing the ratio of amylose in the diet can increase the quantity of starch that flows to the large intestine for microbial fermentation. This leads to the alteration of microbiota and metabolite of the hindgut, where the underlying mechanism is not clearly understood. The present study used a combination of 16S amplicon sequencing technology and metabolomics technique to reveal the effects of increasing ratios of amylose/amylopectin on cecal mucosa- and digesta-associated microbiota and their metabolites in young goats. Twenty-seven Xiangdong black female goats with average body weights (9.00 ± 1.12 kg) were used in this study. The goats were randomly allocated to one of the three diets containing starch with 0% amylose corn (T1), 50% high amylose corn (T2), and 100% high amylose corn (T3) for 35 days. Results showed that cecal valerate concentration was higher (P < 0.05) in the T2 group than those in the T1 and T3 groups. The levels of tumor necrosis factor-α (TNF-α) and interleukin (IL)-6 were decreased (P < 0.05) in cecal tissue while IL-10 was increased (P < 0.05) in the T2 group when compared with T1 or T3 groups. At the phylum level, the proportion of mucosa-associated Spirochaetes was increased (P < 0.05), while Proteobacteria was deceased by feeding high amylose ratios (P < 0.05). The abundance of Verrucomicrobia was decreased (P < 0.05) in the T3 group compared with the T1 and T2 groups. The abundance of digesta-associated Firmicutes was increased (P < 0.05) while Verrucomicrobia and Tenericutes were deceased (P < 0.05) with the increment of amylose/amylopectin ratios. The LEfSe analysis showed that a diet with 50% high amylose enriched the abundance of beneficial bacteria such as Faecalibacterium and Lactobacillus in the digesta and Akkermansia in the mucosa compared with the T1 diet. The metabolomics results revealed that feeding a diet containing 50% high amylose decreased the concentration of fatty acyls-related metabolites, including dodecanedioic acid, heptadecanoic acid, and stearidonic acid ethyl ester compared with the T1 diet. The results suggested that a diet consisting of 50% high amylose could maintain a better cecal microbiota composition and host immune function.

Keywords: amylose/amylopectin ratio; cytokines; goats; metabolite; microbiota.