Role of lncRNA LIPE-AS1 in adipogenesis

Adipocyte. 2022 Dec;11(1):11-27. doi: 10.1080/21623945.2021.2013415.

Abstract

Recent studies have identified long non-coding RNAs (lncRNAs) as potential regulators of adipogenesis. In this study, we have characterized a lncRNA, LIPE-AS1, that spans genes CEACAM1 to LIPE in man with conservation of genomic organization and tissue expression between mouse and man. Tissue-specific expression of isoforms of the murine lncRNA were found in liver and adipose tissue, one of which, designated mLas-V3, overlapped the Lipe gene encoding hormone-sensitive lipase in both mouse and man suggesting that it may have a functional role in adipose tissue. Knock down of expression of mLas-V3 using anti-sense oligos (ASOs) led to a significant decrease in the differentiation of the OP9 pre-adipocyte cell line through the down regulation of the major adipogenic transcription factors Pparg and Cebpa. Knock down of mLas-V3 induced apoptosis during the differentiation of OP9 cells as shown by expression of active caspase-3, a change in the localization of LIP/LAP isoforms of C/EBPβ, and expression of the cellular stress induced factors CHOP, p53, PUMA, and NOXA. We conclude that mLas-V3 may play a role in protecting against stress associated with adipogenesis, and its absence leads to apoptosis.

Keywords: Ceacam1; LIPE; adipogenesis; apoptosis; long non-coding RNA.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adipocytes
  • Adipogenesis* / genetics
  • Adipose Tissue
  • Animals
  • CCAAT-Enhancer-Binding Protein-beta
  • Mice
  • RNA, Long Noncoding* / genetics

Substances

  • CCAAT-Enhancer-Binding Protein-beta
  • RNA, Long Noncoding