BLI-MS: Combining biolayer interferometry and mass spectrometry

Proteomics. 2022 May;22(9):e2100031. doi: 10.1002/pmic.202100031. Epub 2022 Jan 5.


Biolayer interferometry (BLI) is a technology which allows to study the affinity between two interacting macro-molecules and to visualize their kinetic of interaction in real time. In this work, we combine BLI interaction measurement with mass spectrometry in order to identify the proteins interacting with the bait. We provide for the first time the proof of concept of the feasibility of BLI-MS in complex biological mixtures.

Keywords: biolayer interferometry; mass spectrometry; molecular interaction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Interferometry* / methods
  • Kinetics
  • Mass Spectrometry
  • Proteins* / chemistry


  • Proteins