In vivo assessment of molybdenum and cadmium co-induce nephrotoxicity via causing calcium homeostasis disorder and autophagy in ducks (Anas platyrhyncha)

Caiying Zhang; Tianjin Lin; Gaohui Nie; Ruiming Hu; Shaoxing Pi; Zejing Wei; Chang Wang; Guyue Li; Guoliang Hu

doi:10.1016/j.ecoenv.2021.113099

In vivo assessment of molybdenum and cadmium co-induce nephrotoxicity via causing calcium homeostasis disorder and autophagy in ducks (Anas platyrhyncha)

Ecotoxicol Environ Saf. 2022 Jan 15:230:113099. doi: 10.1016/j.ecoenv.2021.113099. Epub 2021 Dec 25.

Authors

Caiying Zhang¹, Tianjin Lin¹, Gaohui Nie², Ruiming Hu¹, Shaoxing Pi¹, Zejing Wei¹, Chang Wang¹, Guyue Li¹, Guoliang Hu³

Affiliations

¹ Jiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University, No. 1101 Zhimin Avenue, Economic and Technological Development District, Nanchang 330045, Jiangxi, PR China.
² School of Information Technology,Jiangxi University of Finance and Economics, No. 665 Yuping West street, Economic and Technological Development District, Nanchang 330032, Jiangxi, PR China.
³ Jiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University, No. 1101 Zhimin Avenue, Economic and Technological Development District, Nanchang 330045, Jiangxi, PR China. Electronic address: hgljx3818@jxau.edu.cn.

PMID: 34963067
DOI: 10.1016/j.ecoenv.2021.113099

Abstract

Excess molybdenum (Mo) and cadmium (Cd) are widespread environmental and industrial metal pollutants. To evaluate the combined effects of Mo and Cd on calcium homeostasis and autophagy in duck kidneys. 160 healthy 7-day-old ducks (Anas platyrhyncha) were randomized into 4 groups and given to a basic diet, adding various doses of Mo or/and Cd for 16 weeks. On the 4th, 8th, 12th and 16th weeks, kidney tissues were collected. The study exhibited that Mo or/and Cd caused histological abnormality, reduced the activities of Ca²⁺ ATPase, Mg²⁺ ATPase, Na⁺-K⁺ ATPase and Ca²⁺-Mg²⁺ ATPase, K and Mg contents, and increased Na and Ca contents, upregulated CaMKKβ, CaMKIIɑ, CaN, IP₃R, GRP78, GRP94, CRT mRNA levels and CaMKIIɑ, CaN, IP₃R protein levels. Moreover, exposure to Mo or/and Cd notably promoted the amount of autophagosomes and LC3II immunofluorescence, upregulated AMPKα1, ATG5, Beclin-1, LC3A, LC3B mRNA levels and Beclin-1, LC3II/LC3I protein levels, downregulated mTOR, Dynein, P62 mRNA levels and P62 protein level. The changes of above indicators in combined group were more obvious. Overall, the results suggest that Mo and Cd co-exposure may can synergistically induce nephrotoxicity via causing calcium homeostasis disorder and autophagy in ducks.

Keywords: Autophagy; Cadmium; Calcium homeostasis; Duck; Molybdenum.