Microcalorimetric titrations of bullfrog (Rana catesbeiana) skeletal troponin C with Ca2+ were carried out in the absence of Mg2+ at 25 degrees C and at pH 7.0. The observed enthalpy titration curve was divided into three stages. The first stage of the titration (up to 2 mol of Ca2+/mol of protein) was characterized as an extremely exothermic process (delta H = -52 kJ/mol of site), the second one (titration from 2 to 3 mol of Ca2+/mol of protein) as a weakly endothermic process (delta H = +26 kJ/mol of site), and the final one (over 3 mol of Ca2+/mol of protein) as a moderately exothermic process (delta H = -35 kJ/mol of site). The endothermic process of Ca2+ binding to the third site (the second stage) has the same property as that of the Ca2+ binding to every site of calmodulin but is distinctly different from those of the calmodulin-trifluoperazine complex and parvalbumins. This may suggest that an endothermic nature of Ca2+ binding, the reaction being driven solely by entropy change, is characteristic of the regulatory reactions of Ca2+ binding proteins accompanying the interaction with other proteins. The third Ca2+ binding site of bullfrog troponin C is, therefore, possibly involved in the regulation of muscle contraction.