Short-term protective effect of octreotide on the lungs of rats with experimentally induced sepsis

Ulus Travma Acil Cerrahi Derg. 2022 Jan;28(1):8-14. doi: 10.14744/tjtes.2020.02589.

Abstract

Background: Acute respiratory distress syndrome is a devastating complication of severe sepsis. Preclinical models suggest that direct lung injury begins with attack to the lung epithelium, but indirect lung injury results from systemic endothelial damage due to inflammatory mediators. The aim of the present study was to explore the effect of octreotide on lungs in a surgically induced sepsis model in rats.

Methods: We used 32 male Sprague Dawley rats and divided into four groups. Group 1: Normal (non-operative and orally fed control, n=8); Group 2: Sham operated (n=8); Group 3: Cecal ligation and puncture (CLP) (untreated group, n=8); and Group 4: CLP and 100 µg/kg octreotide i.p. (n=8). For sepsis, CLP procedure was performed on 16 rats to induce a sepsis model. All groups were analyzed, their blood was taken for arterial blood gas analysis. For histological examination, lung tissues were removed and sections were prepared.

Results: In histological examination, if we compare CLP + Octreotide with only CLP group in CLP + Octreotide group decreased inflammatory cell infiltration in alveolar and interstitial area as well as edema, bleeding, when CLP group was compared with octreotide group, all histopathological parameters improved significantly and the severity index decreased from 3 to 1. For arterial blood gas, when CLP and octreotide groups were compared with CLP group, it was observed that there was a significant change in favor of healing and that they almost came up to controls and sham group.

Conclusion: It could be hypothesized that it would be beneficial to administer octreotide for ameliorate lung injury state in sepsis patients.

MeSH terms

  • Acute Lung Injury*
  • Animals
  • Cecum / surgery
  • Disease Models, Animal
  • Humans
  • Ligation
  • Lung
  • Male
  • Octreotide / pharmacology
  • Rats
  • Rats, Sprague-Dawley
  • Sepsis* / complications
  • Sepsis* / drug therapy

Substances

  • Octreotide