Highly sensitive and quantitative biodetection with lipid-polymer hybrid nanoparticles having organic room-temperature phosphorescence

Biosens Bioelectron. 2022 Mar 1;199:113889. doi: 10.1016/j.bios.2021.113889. Epub 2021 Dec 17.


A versatile organic room-temperature phosphorescence (RTP)-based "turn on" biosensor platform has been devised with high sensitivity by combining oxygen-sensitive lipid-polymer hybrid RTP nanoparticles with a signal-amplifying enzymatic oxygen scavenging reaction in aqueous solutions. When integrated with a sandwich-DNA hybridization assay on 96-well plates, our phosphorimetric sensor demonstrates sequence-specific detection of a cell-free cancer biomarker, a TP53 gene fragment, with a sub-picomolar (0.5 p.m.) detection limit. This assay is compatible with detecting cell-free nucleic acids in human urine samples. Simply by re-programming the detection probe, our unique methodology can be adapted to a broad range of biosensor applications for biomarkers of great clinical importance but difficult to detect due to their low abundance in vivo.

Keywords: Cell-free DNA detection; Enzymatic signal amplification; Lipid-polymer hybrid nanoparticles; Phosphorimetric assay; Purely organic room-temperature phosphorescent nanosensor.

MeSH terms

  • Biosensing Techniques*
  • Humans
  • Lipids
  • Nanoparticles*
  • Polymers
  • Temperature


  • Lipids
  • Polymers