Subcutaneous amyloidoma models for screening potential anti-fibrillating agents in vivo

STAR Protoc. 2021 Dec 14;2(4):101027. doi: 10.1016/j.xpro.2021.101027. eCollection 2021 Dec 17.

Abstract

Here, we describe a robust protocol using mouse models to screen potential insulin-stabilizers and insulin moieties. We have generated a mouse model of amyloidoma, found in diabetic patients undergoing insulin therapy. This model can be used to screen potential insulin stabilizers and insulin moieties to prevent amyloidoma formation. This protocol can further be used for the preclinical validation of therapeutically relevant insulin stabilizers and formulations. The protocol highlights all the critical steps for generating amyloidoma in a preclinical model. For complete details on the use and execution of this profile, please refer to Mukherjee et al. (2021).

Keywords: Health Sciences; Metabolism; Microscopy; Model Organisms; Protein Biochemistry.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amyloid* / chemistry
  • Amyloid* / drug effects
  • Amyloid* / metabolism
  • Amyloidosis* / metabolism
  • Amyloidosis* / pathology
  • Animals
  • Disease Models, Animal*
  • Drug Evaluation, Preclinical / methods*
  • Insulin / chemistry
  • Insulin / metabolism
  • Male
  • Mice
  • Mice, Inbred BALB C

Substances

  • Amyloid
  • Insulin